While Tamil Nadu State Transport Corporation (TNSTC) buses were not operated, private buses were stopped at Zuzuwadi, on the TN border by state police from entering Karnataka to prevent any untoward incidents.
Police prevented entry of private buses fearing attacks by the protesting outfits.
Protests in neighbouring Karnataka also spilled over to Krishnagiri district as hundreds of men, owing allegiance to Kannada Jagurti Vedika and Kannada Rakshana Vedika held a demonstration at Attibelle near Hosur.
The protestors, who came in a rally from the Attibelle bus stand, gathered up for a protest on the interstate border.
Tyres were burnt in the middle of Chennai–Bengaluru national highway disrupting traffic on the stretch.
The protesters led by Kannada Jagurti Vedika K Manjunath Deva shouted slogans against the Goa Chief Minister Manohar Parrikar for failing to secure the rights of the state in the water dispute.
The interstate bus services are likely to resume later in the evening.
The outfits are protesting to put pressure on the state and central governments to resolve the Mahadayi watersharing dispute.
They were protesting the non-implementation of the Kalasa-Banduri dam project, which will divert water from the Mahadayi River to districts in North Karnataka.