The 1,10,000-capacity stadium is the largest cricket stadium in the world in terms of capacity.
The recent India-England Test matches were the first international cricket matches to be held after its reconstruction.
"We are expecting a lot more crowd than what we saw in the Test matches. As of now, we have been able to sell over 40,000 tickets. We are expecting that the seats will be filled up for the first T20 International," said a Gujarat Cricket Association official.
The tickets are being sold online as well as offline at the hosting venue in Motera and also the Sardar Patel Stadium, Navrangpura here.
Meanwhile, the Indian team did not practice on Wednesday as it took a day off whereas the England team did a training session.
The first T20 International takes place on March 12 (Friday) while the remaining ones also take place at the same venue on March 14, 16, 18 and 20.
All matches will begin at 7 p.m.
The T20 International series will be followed by the ODI series in Pune on March 23, 26 and 28.